Regulatory
OmpR

Part:BBa_R0083:Experience

Designed by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)   Group: Antiquity   (2004-01-27)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_R0083

User Reviews

UNIQ6b85abe73cdf0214-partinfo-00000000-QINU


•••••

iGEM Dundee 2013

The function of this part was characterised and quantified in a new salt-sensing device. This OmpR-responsive promoter (BBa_R0083) was linked up to GFP (BBa_E0040) via a ribosome binding site (BBa_B0034). The new composite part (a reporter) (BBa_K1012005) was tested in a E. coli chassis that contained active EnvZ (the sensor kinase that activates OmpR) and compared to tests in an envZ mutant strain. The complete device was found to work well. See BBa_K1012005.


UNIQ6b85abe73cdf0214-partinfo-00000007-QINU